Recombinant IL2 Receptor beta anticorps

N° du produit ABIN7543150

Cet anticorps anti-IL2 Receptor beta est un anticorps Lapin Chimeric détectant IL2 Receptor beta dans FACS, ELISA, IP, BR et InhA. Adapté pour Humain.

Aperçu rapide pour Recombinant IL2 Receptor beta anticorps (ABIN7543150)

Antigène: IL2 Receptor beta (IL2RB) (Interleukin 2 Receptor, beta (IL2RB))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Chimeric

Conjugué: Cet anticorp IL2 Receptor beta est non-conjugé

Application: Flow Cytometry (FACS), ELISA, Immunoprecipitation (IP), Blocking Reagent (BR), Inhibition Assay (InhA)

Clone: Mik-beta 1

Détail du produit anti-IL2 Receptor beta anticorps

Fonction: Anti-IL-2R Beta chain [Mik-beta 1], Rabbit IgG, kappa

Specificité: This antibody recognizes and binds the beta chain of the IL-2 receptor and completely inhibits the IL-2 binding to the beta chain. The beta subunit is involved in receptor mediated endocytosis and transduction of the mitogenic signals of IL2.

Attributs du produit:

Original Species of Ab: Mouse

Original Format of Ab: IgG2a

Purification: Protein A affinity purified

Immunogène: The original antibody was generated by immunizing BLAB/c mice with YTS cells, a human NK-like cell line that highly expresses the human IL-2R beta chain as immunogen.

Isotype: IgG kappa

Information d'application

Indications d'application: Mik-beta 1 inhibited binding of iodine labelled IL-2 to YTS cells in a dose dependent manner. Mik-beta 1 also successfully immunoprecipitated beta chain of the IL-2R from radio iodinated YTS extract. Biotinylated Mik-beta 1 also efficiently competes with IL-2 for binding to solubilized beta chain. Flow cytometry was used to study the cellular disctribution of beta chain, as determined by mik-beta 1 staining. This antibody completely inhibited IL-2 dependent T cell growth in presence of anti-Tac. Two-color flow cytometric analysis using anti-beta chain mAbs clearly showed that approximately 10 % of peripheral blood lymphocytes manifest the beta chain and that this population is mainly composed of Leu-19+ NK cells (PMID: 2467293). Mik beta 1 antibody acts synergistically with humanized anti-Tac to prevent IL-2-induced proliferation of activated T cells (PMID: 8335891). A recombinant single chain immunotoxin of Mik-beta 1 and Pseudomonas derived exotoxin PE40 was selectively cytotoxic for cells expressing high levels of IL-2R beta (p75) subunit (PMID: 1401913).

Commentaires:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Détails sur IL2 Receptor beta

Antigène: IL2 Receptor beta (IL2RB) (Interleukin 2 Receptor, beta (IL2RB))

Autre désignation: IL-2R Beta chain

Sujet: CD122, IL-2R, IL2Rβ, IL-2RB, p70-75, p75, Interleukin-2 receptor subunit beta, IL-2 receptor subunit beta, IL-2R subunit beta, High affinity IL-2 receptor subunit beta, Interleukin-15 receptor subunit beta, Mik-β1

UniProt: P14784

Pathways: Signalistation JAK/STAT, Growth Factor Binding